Drag-brake Mechanism Of A Spindle Motor kinesin-5
نویسندگان
چکیده
منابع مشابه
Kinesin-5 acts as a brake in anaphase spindle elongation
Bipolar kinesin-5 motors, essential in diverse organisms, can generate positive sliding forces between overlapped interpolar microtubules to push mitotic spindle poles apart. BMK-1, the sole Caenorhabditis elegans kinesin-5, is not essential. We have determined, by tracking pole movements in bmk-1 mutant C. elegans embryos, that BMK-1 actually resists pole separation during anaphase. This provi...
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A kinesin-5-dependent 'sliding filament' mechanism is commonly used to actively push apart the poles during mitotic spindle assembly and elongation, but a recent study now shows that, in C. elegans, kinesin-5 is deployed as a brake to slow down spindle-pole separation.
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During mitosis, chromosome passenger complexes (CPCs) exhibit a well-conserved association with the anaphase spindle and have been implicated in spindle stability. However, their precise effect on the spindle is not clear. In this paper, we show, in budding yeast, that a CPC consisting of CBF3, Bir1, and Sli15, but not Ipl1, is required for normal spindle elongation. CPC mutants slow spindle el...
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Kinesin-5 is essential in many species for the formation of a bipolar spindle. Although bipolar tetramers were known to crossbridge pairs of microtubules, the mechanism for organizing spindles was unclear. However, new experiments have revealed unique properties of kinesin-5, including some associated with the tail domain, that provide clues as to how spindles are assembled.
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The kinesin-5 Saccharomyces cerevisiae homologue Cin8 is shown here to be differentially phosphorylated during late anaphase at Cdk1-specific sites located in its motor domain. Wild-type Cin8 binds to the early-anaphase spindles and detaches from the spindles at late anaphase, whereas the phosphorylation-deficient Cin8-3A mutant protein remains attached to a larger region of the spindle and spi...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2009
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2008.12.2615